ABSTRACT
The in-vitro antioxidant activity of Rhaponticum acaule essential oil (RaEO) was evaluated using ß-carotene/linoleic acid bleaching, chelating activity, and lipid peroxidation inhibition (TBARS) assays. The antimicrobial activity of RaEO was assessed by disc diffusion and microdilution methods against 8 bacteria and 4 yeast. Finally, the allelopathic activity of RaEO on the seed germination and the shoot and root elongation of lettuce (Lactuca sativa L.) seedlings were investigated. According to our results, the RaEO exhibited significant antioxidant activity, similar to those of standards (BHT and ascorbic acid) with IC50 values of 0.042 and 0.045 mg/mL obtained by ß-carotene bleaching and TBARS assays, respectively. On the other hand, despite its interesting ferrous chelating activity, RaEO possesses moderate IC50 value (0.35 mg/mL) as compared with that of EDTA (0.015 mg/mL). RaEO exhibited a strong antimicrobial activity against all the tested microorganisms, with IZ, MIC and MBC values being in the range of 7.67 ± 0.58 to 13.33 ± 0.58 mm, 1.25 to 5.00 and 5.00 to 10.00 mg/mL, respectively. The results reveled also that RaEO inhibited the shoot and root growth of Lactuca sativa L. seedlings. Our data suggested that the RaEO had pharmaceutical benefits and could be used as a potential natural herbicide resource
Subject(s)
Asteraceae/classification , Leuzea/classification , Anti-Infective Agents/analysis , Antioxidants/analysis , Oils, Volatile/analysis , Pharmaceutical PreparationsABSTRACT
<p><b>OBJECTIVE</b>To analyze the monosaccharide composition in the polysaccharides from Rhaponticum uniforum, determine the content of monosaccharide, and provide some references for further research.</p><p><b>METHOD</b>The monosaccharide composition was determined by high performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD). Phenol-sulfuric acid method was used for the determination of the content of polysaccharide.</p><p><b>RESULT</b>The monosaccharides composition in polysaccharides from R. uniforum are glucose, arabonose and fructose. Their molar ratios are 1 : 1.61 : 2.21. The content of polysaccharide is 95.78%, taking the mixture of monosaccharide compositions as reference substances.</p><p><b>CONCLUSION</b>HPAEC-PAD can be used to analyze the monosaccharide composition in the polysaccharide with high precision, and the method of phenol-sulfuric acid is simple, convenient and reliable.</p>
Subject(s)
Calibration , Chromatography, High Pressure Liquid , Drugs, Chinese Herbal , Chemistry , Leuzea , Chemistry , Monosaccharides , Polysaccharides , Reproducibility of Results , Sensitivity and SpecificityABSTRACT
<p><b>OBJECTIVE</b>To study the effects of Rhaponticum uniforum polysaccharide on immune function in normal mice and the underlying mechanism.</p><p><b>METHOD</b>SRBC and ovalbumin were employed as antigens to be injected to mice, respectively. Three doses of R. uniforum polysaccharide (50, 100, 200 mg x kg(-1) x d(-1)) were given orally for seven days. After the secondary immunization, the level of corresponding antibody and the concentration of serum IL-2, IFN-gamma were determined.</p><p><b>RESULT</b>The levels of antibodies (anti-SRBC and anti-ovalbumin) and cytokines (IL-2 and IFN-gamma) in median dose group of R. uniforum polysaccharide were all significantly higher than those in control groups (P <0.05).</p><p><b>CONCLUSION</b>R. uniforum polysaccharide could enhance the immune function in normal mice.</p>
Subject(s)
Animals , Male , Mice , Antibodies , Allergy and Immunology , Antibody Formation , Dose-Response Relationship, Drug , Erythrocytes , Allergy and Immunology , Interferon-gamma , Blood , Interleukin-2 , Blood , Leuzea , Chemistry , Ovalbumin , Allergy and Immunology , Plant Roots , Chemistry , Plants, Medicinal , Chemistry , Polysaccharides , Pharmacology , Random Allocation , SheepABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituents of the root of Rhaponticum uniflorum.</p><p><b>METHOD</b>Separation and purification were performed on silica gel and Sephadex LH-20 column chromatography. Their structure were elucidated on the basis of physicochemical and spectral analysis.</p><p><b>RESULT</b>Five triterpenoid compounds were isolated and identified as ursolic acid (1), 3-oxo-19alpha-hydroxyurs-12-en-28-oic acid (2), pomolic acid (3), arjunic acid (4) and tormentic acid (5), respectively.</p><p><b>CONCLUSION</b>Compounds 1 approximately 5 were isolated from the genus Rhaponticum for the first time.</p>